The FruitFast Advantage - Anthocyanin Testing

We decided to test FruitFast Tart Cherry Juice Concentrate against several other brands, using Great Lakes Scientific, a USDA Accredited Laboratory for Chemical Analysis. We purchased competitors bottles of Tart Cherry Juice Concentrate with similar expiration dates and sent them all in the same day for testing. We were surprised at just how much larger the Anthocyanin values were for our concentrate compared to the others tested.


Control/Tracking Number: 09-SA-1654-ACSM

Activity: Scientific Abstract

Current Date/Time: 11/3/2008 4:24:40 PM


The Effect Of Antioxidant Cherryflex® Supplementation On Exercise Induced Doms, Biomarkers Of Tissue Damage, And Oxidative Stress


Author Block: Gary M. Kastello1, Emilene R. Clark1, Sarah N. Ruhl1, Megan C. Bretl1, Jillian A. Mesmer1, Chelsey L. Mitchener1, Lindsey P. McNea1, Jeff D. Strauss1, Courtney L. Delvaux1, Jake D. Hoeppner1, Chris G. Meyer1, Daniel A. Rivera1, Katie M. Schuller1, Jennifer L. Steffen1, Mark S. Sothmann, FACSM2. 1Winona State University, Winona, MN. 2University of South Carolina, Charleston, SC.

Email: [email protected]



Purpose: The purpose of this crossover, double blind study was to examine the effects of CherryFlex supplementation on eccentric exercise induced inflammation, tissue damage, oxidative stress biomarkers and delayed onset muscle soreness (DOMS).

Methods: Four males and ten females (ages: 21 ± 0.76 years; weight: 72.21 ± 3.7 kg) performed five sets of 10 maximal eccentric contractions of the elbow flexor on a Biodex isokinetic dynamometer. The placebo and CherryFlex supplemental groups each ingested tablets 16 days prior to exercise and continued through the study. Venous blood samples were obtained at 16 and 1 day prior to exercise and 0, 2, 4, 24, 48 and 72 hours post exercise. Samples were measured for creatine kinase (CK), myoglobin, protein carbonyls (PC), thiobarbituric acid (TBARS), and C-Reactive proteins (CRP). Data for limb volume, limb girth, arm hang angle, peak torque, peak work, punctuated tenderness gauge (objective pain) and visual analog scale (subjective pain) were collected at 0, 12, 24, 48, and 72 hours post exercise. Time by treatment and treatment effects were measured using MANOVA repeated measures with Tukey’s post-hoc at specific time points.

Results: Moderately significant treatment effect were observed in TBARS (± SE; P24 4.15 ± 0.43 vs. S24 3.42 ± 0.29; p=0.1062), 12 hour objective pain at 4 cm site (P12 1.25 ± 0.13 vs. S12 1.63 ± 0.20; p= 0.139), 12 hour relaxed arm hang angle (P12 145.67 ± 2.15 vs. S12 150.18 ± 1.69; p=0.100) and percent of maximum torque loss (S24 21 ± 4.2 %, P24 29 ± 4.2 %, p = 0.11). Statistically significant time by treatment interactions between groups at p = 0.05, were observed for CRP (P24 5.02 ± 2.02 vs. S24 2.85 ± 0.95; p= 0.047), objective pain (P12 1.41 ± 0.06 vs. S12 1.52 ± 0.08; p= 0.040) and subjective pain (P48 4.86 ± 0.39 vs. S48 4.14 ± 0.41; p= 0.026). The cherry supplemental group demonstrated no significant differences in protein carbonyls, creatine kinase, myoglobin, limb volume, limb girth, flexed arm hang angle, peak torque and peak work.

Conclusions: These findings suggest that ingestion of the CherryFlex supplement prior to and during eccentric exercise may have a protective effect on oxidative stress (TBARS), inflammation (CRP), range of motion, contractile force loss, and perceived pain.


Author Disclosure Information:   G.M. Kastello, Brownwood Acres Inc., Contracted Research.



Control/Tracking Number: 10-SA-1686-ACSM

Activity: Scientific Abstract

Current Date/Time: 11/11/2010 8:21:30 PM


The Effect of CherryFlex Supplementation in Attenuating Eccentric Exercise-Induced Symptoms of DOMS


Author Block: Gary M. Kastello, Haley A. Bawek, Lisa M. Conrad, Korie M. Jackson, Michelle C. Jeske, MiKayla M. Sanocki, Kenzie L. Schleicher, Kendall R. Straessle. Winona State University, Winona, MN. (Sponsor: Mark S. Sothmann, FACSM)

Email: [email protected]



Both untrained and trained individuals often experience post-exercise effects such as delayed onset muscle soreness (DOMS), muscle injury and oxidative stress. The antioxidant,anti-inflammatory and analgesic properties of cherries may provide additional protection from oxidative stress and relieve symptoms of DOMS following exercise.

Purpose: To examine the effect of cherry supplementation in attenuating eccentric exercise-induced symptoms of delayed onset muscle soreness.

Methods: This study was a placebo controlled, repeated measures double-blind, cross-over design. Nineteen college-aged males (20.32+1.45 yrs) participated in this study. Each subject was randomly assigned to ingest either a cherry supplement (CS) or placebo (P) immediately pre-exercise, and 12,24,48,72, and 96 hours post-exercise. Five sets of ten maximal eccentric bicep contractions at 30° .sec-1 with a two minute rest between sets were completed on a Biodex to initially induce muscle damage and inflammation. Data collection was performed immediately prior to exercise and 12, 24, 48, 72, and 96 hours post exercise. Measurements included systolic blood pressure (SBP), punctuated tenderness (PT), visual analog pain scale (VAS), arm girth, arm volume, ROM, serum C-reactive protein (CRP), peak torque, and peak work. Two weeks following the first session, subjects returned to perform a second exercise session with the contralateral limb and ingestion of the opposing treatment. Treatment (TX), time (T), and treatment x time (TX x T) interactions were analyzed using repeated measures ANOVA.

Results: Significant TX effects were observed for PT at 4 cm (mean +SE; CS48 1.39 + 0.15, P48 1.23 + 0.16 kg; p=0.05) and 12 cm (CS48 1.10 + 0.13, P48 0.85 + 0.08 kg; p=0.01). Significant TX effects for VAS were also observed between CS and P groups (CS48 2.94 + 0.41, P48 3.88 + 0.52; p = 0.01). A non-significant trend of lower SBP in the CS group was observed (CS24 117.50 + 2.18, P24 124.75 + 2.57; p = 0.08, T x TX; p=0.06).

Cherry supplementation did not affect arm girth, arm volume, serum CRP, ROM, torque production, and work.

Conclusion: Cherry supplementation immediately preceding, and 12,24,48,72, 96 hrs post strenuous eccentric arm exercise relieves symptoms of pain suggesting an analgesic effect.


Author Disclosure Information:   G.M. Kastello: Contracted Research; Brownwood Acres Foods.

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